Susceptibility of Candida albicans to photodynamic therapy using methylene blue and toluidine blue as photosensitizing dyes.
Identifieur interne : 001110 ( Main/Exploration ); précédent : 001109; suivant : 001111Susceptibility of Candida albicans to photodynamic therapy using methylene blue and toluidine blue as photosensitizing dyes.
Auteurs : RBID : pubmed:22165318English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Coloring Agents, Methylene Blue, Photosensitizing Agents, Tolonium Chloride.
- drug effects : Candida albicans.
- microbiology : Periapical Diseases.
- radiation effects : Candida albicans.
- Microbial Sensitivity Tests, Photochemotherapy.
Abstract
The increased resistance of Candida albicans to antibiotic therapy indicates the need for alternative treatments for oral candidiasis. Photodynamic therapy (PDT) has been researched as an alternative tool to inactivate pathogenic microorganisms. It uses a combination of a photosensitizer and a visible light source. This study evaluated the susceptibility of C. albicans to PDT and compared the efficacy of 100 microg/mL methylene blue (MB) and toluidine blue (TB) as photosensitizers. The light source was Indium-Gallium-Aluminum Phosphide (InGaAIP) laser at 53 J/cm2. Suspensions of 108 cells/mL of C. albicans were subject to PDT for 5 minutes in 96-well plates, then decimal dilutions were plated on Sabouraud Dextrose agar After 48h incubation at 37 degreesC, the number of CFU/mL were obtained and submitted to statistical analysis using Kolmogorov-Smirnov, ANOVA (p<0.0001) and Tukey tests. The results showed that MB or laser irradiation alone did not have statistically significant antifungal activity compared to the positive control group (p> 0. 05). Conversely, the number of viable C. albicans cells was reduced significantly after PDT using MB or mainly TB associated to diode laser irradiation. The data proved the efficacy of PDT against C. albicans cells, regardless of the photosensitizer used.
PubMed: 22165318
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Photodynamic therapy (PDT) has been researched as an alternative tool to inactivate pathogenic microorganisms. It uses a combination of a photosensitizer and a visible light source. This study evaluated the susceptibility of C. albicans to PDT and compared the efficacy of 100 microg/mL methylene blue (MB) and toluidine blue (TB) as photosensitizers. The light source was Indium-Gallium-Aluminum Phosphide (InGaAIP) laser at 53 J/cm2. Suspensions of 108 cells/mL of C. albicans were subject to PDT for 5 minutes in 96-well plates, then decimal dilutions were plated on Sabouraud Dextrose agar After 48h incubation at 37 degreesC, the number of CFU/mL were obtained and submitted to statistical analysis using Kolmogorov-Smirnov, ANOVA (p<0.0001) and Tukey tests. The results showed that MB or laser irradiation alone did not have statistically significant antifungal activity compared to the positive control group (p> 0. 05). Conversely, the number of viable C. albicans cells was reduced significantly after PDT using MB or mainly TB associated to diode laser irradiation. The data proved the efficacy of PDT against C. albicans cells, regardless of the photosensitizer used.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">22165318</PMID><DateCreated><Year>2011</Year><Month>12</Month><Day>13</Day></DateCreated><DateCompleted><Year>2012</Year><Month>01</Month><Day>26</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0326-4815</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>2</Issue><PubDate><Year>2011</Year></PubDate></JournalIssue><Title>Acta odontológica latinoamericana : AOL</Title><ISOAbbreviation>Acta Odontol Latinoam</ISOAbbreviation></Journal><ArticleTitle>Susceptibility of Candida albicans to photodynamic therapy using methylene blue and toluidine blue as photosensitizing dyes.</ArticleTitle><Pagination><MedlinePgn>188-92</MedlinePgn></Pagination><Abstract><AbstractText>The increased resistance of Candida albicans to antibiotic therapy indicates the need for alternative treatments for oral candidiasis. Photodynamic therapy (PDT) has been researched as an alternative tool to inactivate pathogenic microorganisms. It uses a combination of a photosensitizer and a visible light source. This study evaluated the susceptibility of C. albicans to PDT and compared the efficacy of 100 microg/mL methylene blue (MB) and toluidine blue (TB) as photosensitizers. The light source was Indium-Gallium-Aluminum Phosphide (InGaAIP) laser at 53 J/cm2. Suspensions of 108 cells/mL of C. albicans were subject to PDT for 5 minutes in 96-well plates, then decimal dilutions were plated on Sabouraud Dextrose agar After 48h incubation at 37 degreesC, the number of CFU/mL were obtained and submitted to statistical analysis using Kolmogorov-Smirnov, ANOVA (p<0.0001) and Tukey tests. The results showed that MB or laser irradiation alone did not have statistically significant antifungal activity compared to the positive control group (p> 0. 05). Conversely, the number of viable C. albicans cells was reduced significantly after PDT using MB or mainly TB associated to diode laser irradiation. The data proved the efficacy of PDT against C. albicans cells, regardless of the photosensitizer used.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pupo</LastName><ForeName>Yasmine M</ForeName><Initials>YM</Initials><Affiliation>Dentistry School, State University of Ponta Grossa (UEPG), Ponta Grossa, Paraná, Brazil. yasminemendes@hotmail.com</Affiliation></Author><Author ValidYN="Y"><LastName>Gomes</LastName><ForeName>Giovana M</ForeName><Initials>GM</Initials></Author><Author ValidYN="Y"><LastName>Santos</LastName><ForeName>Elizabete B</ForeName><Initials>EB</Initials></Author><Author ValidYN="Y"><LastName>Chaves</LastName><ForeName>Luzia</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Michel</LastName><ForeName>Milton D</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Kozlowski</LastName><ForeName>Vitoldo A</ForeName><Initials>VA</Initials><Suffix>Jr</Suffix></Author><Author ValidYN="Y"><LastName>Gomes</LastName><ForeName>Osnara M M</ForeName><Initials>OM</Initials></Author><Author ValidYN="Y"><LastName>Gomes</LastName><ForeName>Joãdo Carlos</ForeName><Initials>JC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Argentina</Country><MedlineTA>Acta Odontol Latinoam</MedlineTA><NlmUniqueID>8610218</NlmUniqueID><ISSNLinking>0326-4815</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Coloring Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Photosensitizing Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>15XUH0X66N</RegistryNumber><NameOfSubstance>Tolonium Chloride</NameOfSubstance></Chemical><Chemical><RegistryNumber>T42P99266K</RegistryNumber><NameOfSubstance>Methylene Blue</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Candida albicans</DescriptorName><QualifierName MajorTopicYN="Y">drug effects</QualifierName><QualifierName MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Coloring Agents</DescriptorName><QualifierName MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Methylene Blue</DescriptorName><QualifierName MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Periapical Diseases</DescriptorName><QualifierName MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Photochemotherapy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Photosensitizing Agents</DescriptorName><QualifierName MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tolonium Chloride</DescriptorName><QualifierName MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22165318</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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